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Bauhinia forficata link shoot regeneration: histological analysis of organogenesis pathway BABT
Mello,Marcia O.; Melo,Murilo; Appezzato-da-Glória,Beatriz.
Plant regeneration was achieved from cells of callus induced from hypocotyl segments of Bauhinia forficata on half strength Murashige and Skoog culture medium supplemented with several concentrations of BAP. Within 40 days of culture shoot buds formation was observed on callus surface. Calli were then transferred to a same composition culture medium without plant growth regulator in order to induce shoot elongation. Histological studies indicated that in vitro plant regeneration in B. forficata occurred through indirect organogenesis. Meristemoids consisting of small cells with dense cytoplasm and prominent nuclei were randomly distributed throughout the callus surface indicating early stages of shoot bud differentiation. Shoots developed de novo from...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tissue culture; Organogenesis; Anatomy.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000400013
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Histological analysis of the callogenesis and organogenesis from root segments of Curcuma zedoaria Roscoe BABT
Mello,Marcia O.; Melo,Murilo; Appezzato-da-Glória,Beatriz.
Callus was induced from root segments taken from in vitro grown plants of Curcuma zedoaria Roscoe. The explants were cultured on agar-solidified Murashige and Skoog medium supplemented with 13.4muM of alpha-naphthaleneacetic acid and 2.2muM of 6-benzylaminopurine at 25ºC in the dark. Histological analysis revealed that callus was formed from the hypertrophied cortical parenchyma cells of the explant. Some of these cells underwent division while the surrounding cells accumulated starch. Callus was capable of shoot bud regeneration after 70 days when it was transfered to liquid medium of the same composition. After 30 days in liquid medium, buds developed from nodular structures. The adventitious shoots developed extensive root systems when they were placed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adventitious bud; Anatomy; Morphogenesis; Zingiberaceae.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000200014
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Plant-insect interactions: an evolutionary arms race between two distinct defense mechanisms Braz. J. Plant Physiol.
Mello,Marcia O.; Silva-Filho,Marcio C..
In this review, plant-insect interaction is discussed as a dynamic system, subjected to continual variation and change. Plants developed different mechanisms to reduce insect attack, including specific responses that activate different metabolic pathways which considerably alter their chemical and physical aspects. On the other hand, insects developed several strategies to overcome plant defense barriers, allowing them to feed, grow and reproduce on their host plants. This review foccuses on several aspects of this complex interaction between plants and insects, including chemical-derived substances, protein-derived molecules and volatile compounds of plants whereas metabolization, sequestration or avoidance are in turn employed by the insects.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adaptation; Co-evolution; Herbivory.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202002000200001
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Quantificação da micropropagação de Curcuma zedoaria Roscoe Scientia Agricola
Mello,Marcia O.; Amaral,Antônio F. C.; Melo,Murilo.
Curcuma zedoaria Roscoe, planta pertencente à família Zingiberaceae que se propaga vegetativamente, tem sido utilizada amplamente por suas propriedades terapêuticas. O interesse na propagação em massa de plantas medicinais in vitro tem aumentado sensivelmente, pois os métodos convencionais de propagação limitam o potencial de utilização destas plantas. Ápices caulinares foram inoculados em meio de cultura sólido contendo os sais básicos e vitaminas de Murashige & Skoog, suplementado com 2,0 mg L-1 de BAP e 30 g L-1 de sacarose. As culturas foram incubadas em sala de crescimento a temperatura de 25±2°C com fotoperíodo de 16/8 horas de claro/escuro. Transferências e repicagens foram realizadas a cada 40 dias. Os resultados das avaliações feitas...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zedoaria; Cultura de tecido; Produção; Muda.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162000000400016
Registros recuperados: 4
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